Comments on: Detecting edge effects http://www.potencyassay.com/2009/11/09/detecting-edge-effects/ A blog about bioassays, immunoassays, and other potency assays Tue, 11 Oct 2011 08:14:47 +0000 hourly 1 http://wordpress.org/?v=3.1.3 By: Dan http://www.potencyassay.com/2009/11/09/detecting-edge-effects/comment-page-1/#comment-11 Dan Thu, 29 Apr 2010 00:02:46 +0000 http://www.potencyassay.com/?p=45#comment-11 Hi Yi, The software I used to create the heatmaps was SigmaPlot, but any plotting software that can do XYZ pairs should work. No, I don't think adding extra liquid in between the wells will do much. If evaporation is truly a problem (I have never encountered an assay where that is true unless you incubate for a really long time), then you can use a humidity chamber to help somewhat. There are also gas permeable membranes that you can cover your plate with. They don't allow water vapor to pass through, but allows other gases to penetrate. I hope that helps, Dan Hi Yi,

The software I used to create the heatmaps was SigmaPlot, but any plotting software that can do XYZ pairs should work.

No, I don’t think adding extra liquid in between the wells will do much. If evaporation is truly a problem (I have never encountered an assay where that is true unless you incubate for a really long time), then you can use a humidity chamber to help somewhat. There are also gas permeable membranes that you can cover your plate with. They don’t allow water vapor to pass through, but allows other gases to penetrate.

I hope that helps,
Dan

]]> By: Yi http://www.potencyassay.com/2009/11/09/detecting-edge-effects/comment-page-1/#comment-9 Yi Sat, 17 Apr 2010 02:22:37 +0000 http://www.potencyassay.com/?p=45#comment-9 Hello Dan, thank you for this blog. How do you generate the heatmap from your experimental data? Is there a special software? Hello Dan, thank you for this blog. How do you generate the heatmap from your experimental data? Is there a special software?

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